Molecular Characterization and Diversity Analysis viz SSR Markers in Linseed (Linum usitatissimum L.) Germplasm
Abstract
Thirteen linseed genotypes were subjected to study the genetic diversity study. 14 primers revealed a high DNA polymorphism among the genotypes. 12 genotypes produced a total of 150 amplified product. Among these 106 were polymorphic with an average of 69.07 percent between JRF-4 and Gaurav genotypes, minimum similarity (57%) was noticedwhereas maximum similarity was noticed between Pcl-16-2 and Parvati (91%) at the molecular level. The dendrogram constructed from the data revealed four distinct clusters. 30-100 % similarity was observed in linseed genotypes when they were subjected to molecular analys is using SSR markers. 4 clusters were obtained using D2 statistics from this analysis; most diverse genotypes were obtained, namely, JRF-4 and Pratapalsi. These genotypes can be used as parents for future breeding programmes of linseed.