Isolation and Characterization of Heterotrophic and chemo-autotrophic bacteria (nitrifying) from Polluted Pond Water of Raipur District from India
Abstract:
The present investigation was carried out at the Department of Agricultural Microbiology, College of Agriculture Raipur, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.) during the year 2017-2019 on bioremediation of polluted pond water for increasing fish production. In all, 8 treatments were replicated three times and used with Completed Randomized Design (CRD) was inoculation of the fish aquarium with the autotrophic and heterotrophic bacteria from the polluted fish ponds, i.e., T1 (Control), T2 Organic source of N (Urea 2 ppm) + No inoculums, T3 Organic source of N (Urea 2 ppm ) + Composite culture of heterotrophic bacteria, T4 Organic source of N (Urea 2 ppm ) + It was a mixture of the composite culture of autotrophic and heterotrophic bacteria, T5 Organic source of N (Urea 8 ppm) + No inoculums, T6 Organic source of N (Urea 8 ppm) + Composite culture of heterotrophic bacteria, T7 Organic source of N (Urea 8 ppm) + It was a mixture of the composite culture of autotrophic and heterotrophic bacteria and T8 Organic source of N (Urea 12 ppm) + Composite culture of heterotrophic bacteria. This study was carried out in twenty-four aquariums (volume 75x45x30 cm3) containing 70.0litre water under greenhouse conditions. The soil and water samples from polluted pond water were taken for isolation of autotrophic and heterotrophic bacterial isolates, twenty-five samples from different locations of the Raipur area were collected. In this study, the pond water and pond soil pH, EC (µhmos/cm) & TDS (mg/l) range was observed from 7.4 to 8.2, 17×10 to 38×10& 234 to 508, respectively and 7.6 to 8.4, 21×10 to 40×10 and 310 to 545, respectively. In the polluted pond water, inoculation of autotrophic and heterotrophic bacteria positively affected ammonia concentration and heterotrophic bacteria (Micrococcus luteus and Ochrobactrum pituitosum) PS5 and PS16 isolateare performing best results in decreasing the ammonia concentration and increasing the nitrate concentration, respectively.