Effect of Culture Medium on In vitro Callus Induction of Guggul [Commiphora wightii (Arnott)] -An Endangered Medicinally Important Desert Plant

Abstract:

Six types of culture media (MS medium, Woody Plant medium, Nitsch and Nitsch medium, Schenk and Hildebrant medium, White medium and Khudson solution C medium) were primarily used for callus induction from guggul leaf explant. The most active auxin level (2,0 mg / l 2,4-D) of callus induction in leaf explant has been augmented by various culture media. Maximum light pinkish brown, semi-compact callus induction (1.02 g) was observed in Woody Plant Medium with 100% frequency followed by light brown MS medium (0.92 g), compact 100% frequency callus and medium white. Minimum yellow, compact callus proliferation was observed (0.55 g) with 80 per cent frequency in Nitsch and Nitsch medium. Callus induction is inhibited in Schenk and Hildebrant medium and Khudson solution C media).